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目的:研究不同类型脑肿瘤中的p53基因突变与P53蛋白积聚及其相关性。方法:采用聚合酶链反应-单链构象多态性(PCR-SSCP)分析及免疫组化法检测100例脑肿瘤p53基因突变及蛋白表达。结果:p53基因突变率为11%(11/100),其中高恶度胶质瘤为37.5%(6/16),低恶度胶质瘤4.3%(1/23),脑膜瘤6.9%(2/29),转移瘤40.0%(2/5)。P53蛋白表达阳性率为22%(22/100),其中高恶度胶质瘤为62.5%(10/16),低恶度胶质瘤为26.1%(6/23),脑膜瘤10.3%(3/29),转移瘤60%(3/5);其他肿瘤均未发现p53基因突变或蛋白表达。P53蛋白表达阳性的22例中伴有p53基因突变者11例,多见于高恶度肿瘤。结论:p53基因失活在脑肿瘤恶性进展过程中起重要作用。p53基因突变与P53蛋白积聚相关,但并非唯一因素。
Objective: To study the p53 gene mutation and P53 protein accumulation in different types of brain tumors and their correlation. Methods: p53 gene mutation and protein expression in 100 cases of brain tumors were detected by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and immunohistochemistry. Results: The mutation rate of p53 gene was 11% (11/100), of which 37.5% (6/16) in high grade glioma, 4.3% (1/23) in low grade glioma and 6.9% in meningioma 2/29), metastases 40.0% (2/5). The positive rate of P53 protein expression was 22% (22/100), including 62.5% (10/16) in high-grade glioma, 26.1% (6/23) in low-grade glioma, 10.3% 3/29), and 60% (3/5) of metastases. No p53 mutation or protein expression was found in other tumors. Of the 22 cases with P53 protein positive, there were 11 cases of p53 gene mutation, which were more common in high-grade tumors. Conclusion: p53 gene inactivation plays an important role in the malignant progression of brain tumors. p53 gene mutation and P53 protein accumulation related, but not the only factor.