论文部分内容阅读
目的:建立RP-HPLC法同时测定清开灵注射液、板蓝根药材中尿苷、鸟苷、告依春、腺苷的含量。方法:采用Phenomenex C18(250 mm×4.6 mm,5μm)色谱柱,以甲醇(A)-水(B)为流动相进行梯度洗脱,柱温40℃,流速1.0 mL·min-1,检测波长254 nm,进样量1μL。结果:尿苷、鸟苷、(R,S)-告依春和腺苷4个成分质量浓度分别在6.54~261.6μg·mL-1(r=0.9999)、9.42~376.8μg·mL-1(r=0.9999)、7.9~316.0μg·mL-1(r=0.9999)、8.74~349.6μg·mL-1(r=0.9999)的范围内与峰面积呈良好的线性关系;方法的平均回收率(n=6)分别为102.9%、97.2%、103.1%、98.2%。结论:所建立的高效液相色谱测定法简便、准确、重复性好、专属性强,可用于清开灵注射液和板蓝根药材含量测定质量控制。
OBJECTIVE: To establish an RP-HPLC method for simultaneous determination of uridine, guanosine, yingchun and adenosine in Qingkailing Injection and Radix Isatidis. Methods: The gradient elution was carried out on a Phenomenex C18 (250 mm × 4.6 mm, 5 μm) column with methanol (A) -water (B) as the mobile phase at a flow rate of 1.0 mL · min- 254 nm, injection volume 1μL. Results: The concentrations of uridine, guanosine, (R, S) -epicin and adenosine were 6.54-261.6μg · mL-1 (r = 0.9999) and 9.42-376.8μg · mL-1 (r = 0.9999), 7.9 ~ 316.0μg · mL-1 (r = 0.9999), 8.74 ~ 349.6μg · mL-1 (r = 0.9999). The average recoveries n = 6) were 102.9%, 97.2%, 103.1%, 98.2% respectively. Conclusion: The established HPLC method is simple, accurate, reproducible and specific. It can be used for quality control of Qingkailing Injection and Radix Isatidis.