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目的预测结核分枝杆菌Rv3407的抗原表位。方法利用DNAStar软件包中Protean软件对Rv3407氨基酸序列进行分析,采用包括二级结构、亲水性、抗原性、表面可能性、柔韧性等多参数预测其二级结构及T细胞和B细胞抗原表位。结果 Rv3407蛋白具有丰富的二级结构和多处抗原指数较高的区段,潜在的B细胞抗原表位较少,可能位于11-26、28-43、50-61、66-74、76-99位氨基酸残基或其附近,这些区域基本上含有β转角结构,亲水性、表面可能性和柔韧性指数都较高。该蛋白潜在的T细胞抗原表位较多,可能位于2-12、22-26、34-37、40-44、56-60、75-79、86-93位氨基酸残基或其附近。结论结核分枝杆菌Rv3407是一个T细胞抗原表位占优势的蛋白抗原,B细胞抗原表位略少,预测结果为该蛋白抗原表位的进一步研究与应用奠定了基础。
Objective To predict the antigenic epitope of Mycobacterium tuberculosis Rv3407. Methods The amino acid sequence of Rv3407 was analyzed by Protean software in DNAStar package. The secondary structure, T-cell and B-cell epitopes were predicted by multiple parameters including secondary structure, hydrophilicity, antigenicity, surface potential and flexibility Bit. Results The Rv3407 protein was rich in secondary structure and multiple segments with high antigen index. The potential B cell epitopes were fewer, probably located in 11-26, 28-43, 50-61, 66-74 and 76- At or near the 99th amino acid residue, these regions contain essentially beta-turn structures with high hydrophilicity, surface potential, and flexibility index. Potential T-cell epitopes of the protein are more likely to be located at or near amino acid residues 2-12, 22-26, 34-37, 40-44, 56-60, 75-79, 86-93. Conclusion Mycobacterium tuberculosis Rv3407 is a protein antigen with predominant T cell epitopes, and the B cell epitopes are slightly less. The predicted results provide the basis for further research and application of antigenic epitopes.