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目的探讨c-Jun氨基末端激酶选择性抑制剂(SP600125)对哮喘小鼠肺组织白介素-6(IL-6)表达的影响。方法 30只BALB/c小鼠随机分为对照组、哮喘组和SP600125组,制作哮喘模型及干预处理后,处死小鼠,取肺组织,采用免疫组织化学方法和western blot方法检测各组小鼠肺组织内IL-6的表达。结果免疫组化结果显示,哮喘组小鼠肺组织IL-6表达的平均光密度值为(0.71±0.11),显著高于对照组(0.23±0.03),P<0.01;而SP600125组小鼠肺组织IL-6表达的平均光密度值为(0.46±0.07),与哮喘组相比明显降低,P<0.01。western blot结果显示,哮喘组小鼠肺组织IL-6表达的平均光密度值为(0.62±0.09),显著高于对照组(0.16±0.02),P<0.01;而SP600125组小鼠肺组织IL-6表达的平均光密度值为(0.37±0.05),与哮喘组相比明显降低,P<0.01。结论 c-Jun氨基末端激酶选择性抑制剂能降低哮喘小鼠肺组织IL-6的表达。
Objective To investigate the effect of c-Jun N-terminal kinase selective inhibitor (SP600125) on the expression of interleukin-6 (IL-6) in the lung tissue of asthmatic mice. Methods Thirty BALB / c mice were randomly divided into control group, asthma group and SP600125 group. After asthma and asthma were prepared and treated, mice were sacrificed and lungs were harvested. Immunohistochemistry and western blotting were used to detect the expression of IL- IL-6 expression in lung tissue. Results The results of immunohistochemistry showed that the mean optical density of IL-6 in asthmatic mice was (0.71 ± 0.11), significantly higher than that of the control group (0.23 ± 0.03), P <0.01; while the SP600125 mice lung The average optical density of IL-6 expression was (0.46 ± 0.07), which was significantly lower than that of asthma group (P <0.01). The results of western blot showed that the average optical density of IL-6 in asthmatic mice was (0.62 ± 0.09), significantly higher than that in control group (0.16 ± 0.02), P <0.01; while the SP600125 mice lung IL -6 was (0.37 ± 0.05), which was significantly lower than that in asthma group (P <0.01). Conclusion c-Jun N-terminal kinase selective inhibitor can reduce the expression of IL-6 in lung tissue of asthmatic mice.