论文部分内容阅读
目的研究山茱萸果核水提取物(AEFCOC)的降压作用及其作用机制。方法 2K2C法建立大鼠肾性高血压模型;用大鼠离体胸主动脉环灌流,记录张力变化。结果 AEFCOC可剂量依赖性降低大鼠血压水平;0.03,0.1,0.3,1,2mg·mL-1AEFCOC可以剂量依赖性地舒张PE预收缩的内皮完整的大鼠胸主动脉环;NO合酶抑制剂L-NAME(1×10-4mol·L-1)、环氧酶抑制剂Indo(1×10-5mol·L-1)处理血管后,AEFCOC的舒张血管效应均被阻断(P<0.05);去内皮时,AEFCOC(2 mg·mL-1)对PE预收缩的血管环始呈现舒张作用(P<0.05)。结论 AEFCOC可显著降低大鼠肾性高血压,可能与舒血管有关;其舒血管作用主要是内皮依赖性的,通过NO-鸟苷酸环化酶途径和环氧酶途径。
Objective To study the antihypertensive effect and its mechanism of Fructus Corni fruit water extract (AEFCOC). Method 2K2C established rat model of renal hypertension; isolated rat thoracic aortic rings, recording changes in tension. Results AEFCOC reduced blood pressure in a dose-dependent manner. 0.03, 0.1, 0.3, 1, 2 mg · mL-1AEFCOC could depress the pre-contracted endothelium-intact thoracic aortic rings in a dose-dependent manner. NO synthase inhibitor Vasodilation of AEFCOC was blocked by L-NAME (1 × 10-4 mol·L-1) and cyclooxygenase inhibitor Indo (1 × 10-5 mol·L-1), respectively (P <0.05) At the endothelium, AEFCOC (2 mg · mL-1) exerted a relaxing effect on the pre-contracted vascular rings (P <0.05). Conclusion AEFCOC can significantly reduce renal hypertension in rats, which may be related to vasodilation; its vasodilator effect is mainly endothelium-dependent through NO-guanylate cyclase pathway and cyclooxygenase pathway.