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目的探讨术中快速乙酰胆碱酯酶(AChE)染色法诊断先天性巨结肠(Hirschsprung’sdisease,HD)和肠神经元性发育异常(Intestinalneuronaldysplasia,IND)的可行性及临床意义。方法选择10例诊为HD并行手术治疗的患儿,术中切取病变肠段及吻合口处肠壁组织分别行快速及传统AChE染色检查,比较其诊断结果。结果两种染色方法相比无显著性差异。结论快速AChE染色法可行,是术中检查诊断HD和IND的有效辅助手段。
Objective To investigate the feasibility and clinical significance of intraoperative rapid acetylcholinesterase (AChE) staining in the diagnosis of Hirschsprung’s disease (HD) and intestinal intestinal metaplasia (IND). Methods Ten children diagnosed as HD concurrent surgery were selected. The bowel segments of the intestine and the anastomosis of the lesion were removed and examined by fast and traditional AChE staining respectively. The diagnostic results were compared. Results There was no significant difference between the two staining methods. Conclusion The rapid AChE staining is feasible and effective in the diagnosis of HD and IND during intraoperative examination.