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目的探讨异体异位移植睾丸中生精细胞退化的可能机制。方法以新生1～2 d小鼠睾丸组织为供体,免疫缺陷的雄性去势裸鼠为受体进行组织移植,分别在移植后不同时间(3 d和1～8周共9个时间段)收集移植物组织,提取移植物总RNA,采用半定量RT-PCR检测不同发育阶段移植物中凋亡相关基因fas、bcl-2、bax和caspase3以及DNA损伤修复基因xrcc1、ogg1、ercc1、brca1和brca2的表达,并与相应年龄段正常小鼠睾丸组织中的基因表达进行比较分析。结果与正常发育的小鼠睾丸组织相比,小鼠睾丸移植物中生精细胞退化增加,凋亡相关基因bcl-2表达降低,fas、bax和caspase3表达增加;DNA损伤修复基因xrcc1、ogg1、ercc1、brca1和brca2的表达量均有不同程度的降低。结论新生小鼠睾丸组织移植到免疫缺陷裸鼠体内后,生精细胞退化增加,这可能与促凋亡基因表达增加和抑制凋亡基因表达降低,以及DNA损伤修复基因表达降低有关。
Objective To investigate the possible mechanism of degeneration of spermatogenic cells in allotransplanted testes. Methods The testicular tissues of neonatal mice were used as donor and immunodeficient male castrated nude mice were used as recipients for tissue transplants. The rats were sacrificed at different time after transplantation (9 days at 3 days and 1 week to 8 weeks) The total RNA was extracted and the fas, bcl-2, bax and caspase3, as well as DNA damage repair genes xrcc1, ogg1, ercc1, brca1 and brca2, and compared with the corresponding gene expression in normal mouse testis tissue. Results Compared with the normal mouse testis, the degeneration of spermatogenic cells in mouse testicular grafts increased, the expressions of apoptosis related genes bcl-2 decreased, fas, bax and caspase3 increased. The DNA damage repair genes xrcc1, ogg1, The expression levels of ercc1, brca1 and brca2 all decreased to some extent. Conclusions The degeneration of spermatogenic cells in testis of neonatal mice transplanted into immunodeficient nude mice may be related to the increase of the expression of pro-apoptotic genes, the decrease of the expression of apoptosis-suppressing genes and the decrease of gene expression of DNA damage repair.