论文部分内容阅读
应用荧光光谱法和紫外光谱法对二苯乙烯类化合物白皮杉醇(Piceatannol)、白藜芦醇(Resveratrol)和赤松素(Pinosylvin)与BSA之间的相互作用进行研究.计算了这3种化合物与BSA的结合常数和结合位点数及热力学函数△G,△S,△H.并分析了它们对BSA的荧光淬灭过程及其之间的相互作用类型,运用了Foerster’s偶极-偶极非辐射能量转移原理测定了与牛血清白蛋白的结合距离.结果表明,白皮杉醇、白藜芦醇和赤松素3种二苯乙烯化合物对BSA的荧光淬灭属于自发的以疏水作用力为主导的非辐射能量转移的静态淬灭过程.随分子中B环上的羟基数目的逐渐增加,白皮杉醇、白藜芦醇和赤松素与BSA的结合力逐渐增强.
The interaction between stilbene compounds Piceatannol, Resveratrol, Pinosylvin and BSA was studied by fluorescence spectroscopy and UV spectrophotometry. The binding constants, binding sites and thermodynamic functions △ G, △ S, △ H of the compounds with BSA were analyzed. The fluorescence quenching process and the interaction between BSA and BSA were analyzed. Foerster’s dipole-dipole The binding distance to bovine serum albumin (BSA) was determined by the non-radiative energy transfer principle.The results showed that the fluorescence quenching of three stilbene compounds of piceatan, resveratrol and sebacic was spontaneous and the hydrophobic interaction force was Leading to the static quenching process of non-radiative energy transfer.With the gradual increase of the number of hydroxyl groups on the B ring in the molecule, the binding power of the white-coated paclitaxel, resveratrol and the red pine solid with BSA gradually increases.