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了解烫伤后肠粘膜微血管形态测量学方面的改变,探讨肠粘膜损伤的机制。以大鼠烫伤为模型,用AKP染色分别显示未烫伤及烫伤鼠回肠壁微血管,并进行体视学测量分析。伤后平均截面面积和平均周长均变小。对照组微血管平均截面面积为56.06±8.03μm2,伤后12 h最低(30.90±6.46μm2,P<0.01);对照组血管平均周长为23.84±4.17μm,伤后6 h降至最低,为19.43±1.59μm(P<0.01)。微血管长度密度除伤后12 h组外,其余各组均较对照组高;在伤后12 h肠壁微血管体积密度降低(P<0.01),其余各组虽较对照组高,但无统计学意义(P>0.05)。结果表明大鼠严重烫伤后肠壁微血管管径缩小,证明存在肠血流量减少的现象,旁证肠粘膜损伤是因为缺血缺氧所致。
To understand the morphological changes of intestinal mucosa after scald, and to explore the mechanism of intestinal mucosal injury. Rat scald was used as a model, AKP staining showed non-scalded and scalded mouse ileal wall capillaries, and stereological measurement analysis. After injury, the average cross-sectional area and average circumference became smaller. The average cross-sectional area of microvessels in control group was 56.06 ± 8.03μm2, the lowest was 12h after injury (30.90 ± 6.46μm2, P <0.01). The average perimeter of blood vessels in control group was 23.84 ± 4.17μm, the lowest was 6 h ± 1.59 μm (P <0.01). The microvessel density was higher than that of the control group except 12 h after injury. The volume density of the microvessel was decreased 12 h after injury (P <0.01), while the other groups were higher than the control group, but no statistics was found Significance (P> 0.05). The results showed that the diameter of the intestinal microvessel decreased after severe scald in rats, demonstrating the decrease of intestinal blood flow. The evidence of intestinal mucosal injury was due to ischemia and hypoxia.