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目的探讨灵芝多糖对动脉粥样硬化防治作用的可能机制。方法 SD大鼠给予高脂饲料加腹腔注射维生素D3复制动脉粥样硬化模型,全自动生化仪检测血清血脂含量,透射电子显微镜观察主动脉形态,ELISA法检测C反应蛋白(CRP)及肿瘤坏死因子-α(TNF-α)、Western Blot法检测血管细胞黏附分子-1(VCAM-1)的蛋白表达,观察灵芝多糖对动脉粥样硬化大鼠相关炎症因子表达的影响。结果与模型组相比,灵芝多糖高剂量组可明显降低血清低密度脂蛋白(LDL)、总胆固醇(TC)水平(P<0.01);病理形态显示灵芝多糖组可明显抑制大鼠主动脉斑块形成;灵芝多糖高剂量组血清CRP及TNF-α水平与模型组相比明显下降(P<0.05),并显著下调VCAM-1蛋白表达(P<0.01)。结论灵芝多糖对大鼠动脉粥样硬化具有一定的治疗作用,其机制可能与降低相关炎症因子表达有关。
Objective To explore the possible mechanism of Ganoderma lucidum polysaccharides on the prevention and treatment of atherosclerosis. Methods SD rats were given high-fat diet plus intraperitoneal injection of vitamin D3-induced atherosclerosis model, serum lipid levels were detected by automatic biochemical analyzer. Morphology of the aorta was observed by transmission electron microscopy. C-reactive protein (CRP) and tumor necrosis factor (TNF-α) and Western Blot to detect the expression of vascular cell adhesion molecule-1 (VCAM-1), and to observe the effect of Ganoderma lucidum polysaccharides on the expression of inflammatory cytokines in atherosclerotic rats. Results Compared with the model group, the high dose Ganoderma lucidum polysaccharide group could significantly reduce the levels of serum LDL and TC (P <0.01). The pathological results showed that Ganoderma lucidum polysaccharide group could significantly inhibit aortic plaque (P <0.05), and significantly decreased the expression of VCAM-1 protein (P <0.01) .Conclusion: Ganoderma lucidum polysaccharide high-dose group can significantly reduce the levels of serum CRP and TNF-α. Conclusion Ganoderma lucidum polysaccharide has a certain therapeutic effect on atherosclerosis in rats, and its mechanism may be related to the decrease of the expression of related inflammatory cytokines.