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目的:探讨枳壳健胃颗粒对乙醇致胃粘膜损伤大鼠细胞生长因子的影响。方法:大鼠随机分为正常组、模型组、枳壳健胃颗粒低剂量组(2.7 g/kg)、枳壳健胃颗粒中剂量组(5.4 g/kg)、枳壳健胃颗粒高剂量组(10.8 g/kg)和雷尼替丁组(0.027 g/kg)。枳壳健胃颗粒组给予相对应的剂量,正常组与模型组给予等量的生理盐水,每天1次,连续灌胃15d。末次灌胃给药后1h,除正常组外,其余各组大鼠用75%乙醇制备大鼠胃粘膜损伤模型。放射免疫法测定胃粘膜组织中表皮生长因子(Epidermal Growth Factor,EGF)、转化生长因子α(Transforming Growth Factor-α,TGF-α)的含量。实时荧光定量PCR法(Real-time PCR)检测胃粘膜损伤周围组织中EGF、TGF-α以及表皮生长因子受体(Epithelial Growth Factor Receptor,EGFR)mRNA的表达。结果:与模型组相比较,枳壳健胃颗粒5.4g/kg和10.8g/kg能明显升高大鼠胃粘膜组织中的EGF、TGF-α含量;同时,使大鼠胃粘膜组织中的EGF、TGF-α以及EGFR mRNA表达增强。结论:枳壳健胃颗粒可能通过激活EGF、TGF-α/EGFR系统,促进胃粘膜上皮细胞增生达到修复胃粘膜组织的目的。
Objective: To investigate the effect of Fructus aurantii particles on gastric cancer cell growth factor induced by ethanol in rats. Methods: The rats were randomly divided into normal group, model group, low dose Fructus Aconitum Fructus Granule group (2.7 g / kg), Fructus Astragali Granule medium dose group (5.4 g / kg) Group (10.8 g / kg) and ranitidine group (0.027 g / kg). Fructus Aurajianwei granule group given the corresponding dose, normal group and model group were given the same amount of saline once a day for 15 days. One hour after the last gavage, except the normal group, rats in the other groups were given gastric ulcer model with 75% ethanol. The levels of Epidermal Growth Factor (EGF) and Transforming Growth Factor-α (TGF-α) in gastric mucosa were measured by radioimmunoassay. Real-time PCR was used to detect the mRNA expression of EGF, TGF-α and Epithelial Growth Factor Receptor (EGFR) in the surrounding tissues of gastric mucosa. Results: Compared with the model group, the Fructus Aurajian Weiji particles 5.4g / kg and 10.8g / kg significantly increased the contents of EGF and TGF-α in the gastric mucosa of rats, meanwhile, EGF , TGF-α and EGFR mRNA expression increased. Conclusion: Fructus Aurajianwei granule can repair gastric mucosa by activating EGF, TGF-α / EGFR system and promoting the proliferation of gastric epithelial cells.