论文部分内容阅读
目的:观察复方乌金汤对急性肝损伤大鼠炎症因子TNF-α、IL-1β和HMGB1的影响。方法:SD大鼠分为正常组、模型组、水飞蓟素组和中药组,采用腹腔注射LPS和D-GalN混合液,制备急性肝损伤模型,分别于造模后6、24和48 h处死,检测各组大鼠血清TNF-α和IL-1β含量和肝组织HMGB1表达。结果:模型组各时间点TNF-α、IL-1β和HMGB1均比正常组明显升高(P<0.05或P<0.01);与模型组比较,复方乌金汤中药治疗组血清TNF-α在6、24和48 h均降低(P<0.05或P<0.01),IL-1β在24 h降低(P<0.05),肝脏HMGB1表达在6h和48 h较模型组下降(P<0.01)。结论:复方乌金汤治疗急性肝损伤的作用与其降低炎症因子相关。
Objective: To observe the effects of Fufang Wujin Decoction on inflammatory cytokines TNF-α, IL-1β and HMGB1 in acute liver injury rats. Methods: Sprague-Dawley rats were divided into normal group, model group, silymarin group and Chinese herbal medicine group. The model of acute liver injury was induced by intraperitoneal injection of LPS and D-GalN mixture. The rats were sacrificed at 6, 24 and 48 h after model establishment, The content of TNF-α and IL-1β in serum and the expression of HMGB1 in liver tissues of rats in each group. Results: The levels of TNF-α, IL-1β and HMGB1 in model group were significantly higher than those in normal group at each time point (P <0.05 or P <0.01). Compared with model group, the serum TNF- , 24 h and 48 h (P <0.05 or P <0.01), IL-1β decreased at 24 h (P <0.05), while the expression of liver HMGB1 decreased at 6 h and 48 h (P <0.01). Conclusion: The effect of Fufang Wujin Decoction in treating acute liver injury is related to the reduction of inflammatory factors.