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目的研究不同剂量苯肽胺酸28 d经口染毒对小鼠主要免疫器官脾脏和胸腺组织的氧化损伤作用。方法选用40只成年雄性Balb/c小鼠,随机分成4组,每组10只。分别为对照组,30、100和300 mg/kg苯肽胺酸剂量组,每天灌胃染毒,连续28 d。末次染毒24 h后,检测主要器官脏器系数,苏木素-伊红(HE)染色观察脾脏和胸腺组织病理学变化,分别测定脾脏和胸腺的丙二醛(MDA)含量、总超氧化物歧化酶(T-SOD)和谷胱甘肽过氧化物酶(GSH-Px)的活力。结果连续染毒28 d,苯肽胺酸300 mg/kg剂量组小鼠脾脏脏器系数与对照组相比明显增大(P<0.01),且发生了病理改变。100和300 mg/kg剂量组小鼠脾脏MDA含量明显升高(P<0.05),300 mg/kg剂量组小鼠胸腺GSH-Px活力明显降低(P<0.05),脾脏和胸腺中T-SOD活力的改变没有统计学意义(P>0.05)。结论连续染毒28 d,300 mg/kg苯肽胺酸对小鼠主要免疫器官脾脏和胸腺产生了不同程度的氧化损伤作用。
OBJECTIVE To study the oxidative damage of spleen and thymus in the major immune organs of mice induced by different doses of Phetidine for 28 days. Methods Forty adult male Balb / c mice were randomly divided into four groups (n = 10). Respectively, the control group, 30, 100 and 300 mg / kg phenytidine dose group, intragastric administration of exposure for 28 days. Twenty-four hours after the last exposure, the organ coefficients of major organs were detected. The pathological changes of spleen and thymus were observed by hematoxylin-eosin (HE) staining. The contents of malondialdehyde (MDA), total superoxide dismutase Enzyme (T-SOD) and glutathione peroxidase (GSH-Px) activity. Results The organ coefficient of spleen in the group treated with 300 mg / kg of phenytamine increased significantly (P <0.01), and the pathological changes occurred. (P <0.05). The activity of GSH-Px in the thymus of 300 mg / kg dose group was significantly decreased (P <0.05), while the levels of T-SOD in spleen and thymus No significant change in vitality (P> 0.05). Conclusions Continuous treatment of 28 d, 300 mg / kg of PYT can induce different degrees of oxidative damage to the spleen and thymus of the major immune organs in mice.