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目的观察UVB照射对SLE患者的外周血单个核细胞(PBMC)的自噬现象,探讨UVB、自噬对SLE产生的影响。方法应用RT-q PCR,Western blotting技术在基因和蛋白水平测定活动性SLE患者外周血淋巴细胞自噬标志物Uvrag,Beclin1和LC3Ⅱ的表达水平,并通过统计学分析其内在联系,探讨自噬在SLE发病中的作用机制。结果 RT-q PCR显示:SLE患者外周血单个核细胞接受UVB照射后Uvrag,Beclin1和LC3ⅡmRNA的表达水平较照射前明显升高(P<0.05);SLE患者外周血单个核细胞接受UVB照射后LC3ⅡmRNA的表达水平明显高于正常对照组接受UVB照射的水平(P<0.01)。Western blotting显示:SLE患者的外周血单个核细胞接受UVB照射组较SLE患者组的外周血单个核细胞中的Uvrag,Beclin1和LC3Ⅱ的蛋白水平升高,Uvrag,Beclin1差异有统计学意义(P<0.05);SLE患者的外周血单个核细胞接受UVB照射组较正常人的外周血单个核细胞接受UVB照射组的Uvrag,Beclin1和LC3Ⅱ的蛋白水平轻度升高,LC3Ⅱ差异有统计学意义(P<0.05);SLE患者的外周血单个核细胞在接受UVB照射组较正常未照射UVB组的外周血单个核细胞中的Uvrag,Beclin1和LC3Ⅱ蛋白水平升高(P<0.05)。结论活动性SLE患者外周血单个核细胞中存在自噬现象,UVB能够引发SLE患者发生异常自噬,自噬相关基因/蛋白参与了SLE外周血单个核细胞异常自噬的发生。
Objective To observe the autophagy of peripheral blood mononuclear cells (PBMC) in peripheral blood mononuclear cells (PBMCs) from patients with SLE under UVB irradiation and to explore the effect of UVB and autophagy on the production of SLE. Methods RT-q PCR and Western blotting were used to detect the expression of Uvrag, Beclin1 and LC3Ⅱ in the peripheral blood lymphocytes of patients with active SLE at gene and protein level. The relationship between autophagy and Mechanism of SLE pathogenesis. Results The RT-qPCR results showed that the expression of Uvrag, Beclin1 and LC3ⅡmRNA in peripheral blood mononuclear cells of SLE patients was significantly higher than that before irradiation (P <0.05). In peripheral blood mononuclear cells of SLE patients, the expression of LC3ⅡmRNA Was significantly higher than that of the control group receiving UVB irradiation (P <0.01). Western blotting showed that the protein levels of Uvrag, Beclin1 and LC3Ⅱ in peripheral blood mononuclear cells of peripheral blood mononuclear cells in SLE patients were significantly higher than those of SLE patients in UVB irradiation group, and the difference of Uvrag and Beclin1 was statistically significant (P < 0.05). The protein levels of Uvrag, Beclin1 and LC3Ⅱwere slightly increased in peripheral blood mononuclear cells of peripheral blood mononuclear cells of patients with SLE compared with that of normal controls. LC3Ⅱhad statistical significance (P <0.05). The levels of Uvrag, Beclin1 and LC3Ⅱ protein in peripheral blood mononuclear cells of SLE patients were significantly higher than those of UVB-exposed peripheral blood mononuclear cells (P <0.05). Conclusions Autophagy is present in peripheral blood mononuclear cells of patients with active SLE. UVB can induce abnormal autophagy in SLE patients and autophagy-related genes / proteins are involved in the abnormal autophagy in peripheral blood mononuclear cells of SLE patients.