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目的研究ＶＰ１６诱导ＨＬ－６０急性早幼粒白血病细胞程序化死亡。方法用４种不同浓度的ＶＰ１６诱导ＨＬ－６０细胞程序化死亡，ＤＮＡ电泳、电镜及苔盼蓝活细胞拒染试验测定实验结果。结果４种浓度ＶＰ１６均能诱导ＨＬ－６０细胞程序化死亡。结论ＶＰ１６是一种稳定可靠的细胞凋亡诱导剂。苔盼蓝拒染试验不能用于判定细胞是否发生了程序化死亡。ＤＮＡ电泳是确定细胞程序化死亡的简单可靠的方法。
Objective To investigate the programmed death induced by VP16 in HL-60 acute promyelocytic leukemia cells. Methods The programmed death of HL-60 cells was induced by four different concentrations of VP16. The experimental results were determined by DNA electrophoresis, electron microscopy and trypan blue exclusion test. Results All four concentrations of VP16 could induce apoptosis in HL-60 cells. Conclusion VP16 is a stable and reliable inducer of apoptosis. The Trypan blue exclusion test cannot be used to determine if a programmed cell death has occurred. DNA electrophoresis is a simple and reliable method for determining programmed cell death.