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应用肿瘤内照射和肿瘤表面照射两种方法在人肺腺癌裸小鼠移植瘤(lung adenocarcinoma xenograft,LAX-83)进行光动力学治疗(photodynamic therapy,PDT)的实验研究。结果表明PDT后LAX-83生长延缓。治疗后10天,肿瘤内照射组和肿瘤表面照射组之间的肿瘤体积和重量无显著差异。而在治疗组与对照组、单独激光组和单独光敏剂组之间存在显著差异。形态学观察结果表明肿瘤内照射组的病理改变比肿瘤表面照射组出现更早、更显著。后者肿瘤坏死深度在4～5mm左右,而前者则达10mm以上。光镜下发现肿瘤细胞胞浆、胞核浓缩,肿瘤细胞坏死。电镜检查提示线粒体受损最早。单独注射光敏剂和单独激光照射后均不产生肿瘤组织的显微结构和超微结构的改变。本实验证实PDT对人肺腺癌细胞的杀伤作用,为肿瘤内照射治疗的临床应用提供有益参考。
The photodynamic therapy (PDT) of lung adenocarcinoma in nude mice (Lung adenocarcinoma xenograft, LAX-83) was studied by intratumoral irradiation and tumor surface irradiation. The results showed that LAX-83 growth retarded after PDT. Ten days after treatment, there was no significant difference in tumor volume and weight between the intratumoral irradiation group and the tumor surface irradiation group. There was a significant difference between the treatment group and the control group, the laser alone group, and the photosensitizer alone group. The morphological observations showed that the pathological changes in the intratumoral irradiation group appeared earlier and more significantly than the tumor surface irradiation group. The latter tumor necrosis depth of 4 ~ 5mm, while the former is more than 10mm. Under the light microscope, the cytoplasm and nucleus of the tumor cells were concentrated, and the tumor cells were necrotic. Electron microscopy revealed the earliest mitochondrial damage. Both the injection of photosensitizer alone and laser irradiation alone did not produce changes in the microstructure and ultrastructure of the tumor tissue. This experiment confirmed the killing effect of PDT on human lung adenocarcinoma cells and provided a useful reference for the clinical application of intratumoral irradiation therapy.