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目的:探讨转染人前列腺癌PC-3细胞pEGFP-N1基因的最佳转染方法。方法:以超声微泡造影剂、超声辐照、脂质体转染及其相互结合的方法,将质粒pEGFP-N1基因转染人前列腺癌PC-3细胞,24h后以荧光显微镜观察前列腺癌PC-3细胞中的绿色荧光蛋白表达情况,并用流式细胞仪测定转染率。结果:以超声+微泡+脂质体组基因转染效率最高,与其他组比较,差异均有统计学意义(P<0.05)。结论:超声联合微泡与脂质体结合能明显提高pEGFP-N1基因在人前列腺癌细胞中的转染率,是一种较理想的基因转染方法。
Objective: To investigate the optimal transfection method of pEGFP-N1 gene in human prostate cancer PC-3 cells. Methods: The plasmid pEGFP-N1 gene was transfected into human prostate cancer PC-3 cells with ultrasonic microbubble contrast agent, ultrasound irradiation, liposome transfection and their combination method. After 24 hours, the PC of prostate cancer PC -3 cells in the expression of green fluorescent protein, and using flow cytometry to determine the transfection rate. Results: The efficiency of transfection with ultrasound + microbubbles + liposomes was the highest, compared with other groups, the difference was statistically significant (P <0.05). Conclusion: Ultrasound combined with microbubbles and liposomes can significantly improve the transfection efficiency of pEGFP-N1 gene in human prostate cancer cells, which is an ideal method of gene transfection.