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目的应用多重PCR检测方法对上海市儿童呼吸道病毒感染进行检测,获得疾病谱分析其流行规律。方法 2009年1月到2010年3月上海市儿童医院因呼吸道感染住院病人下呼吸道标本519份,利用多重PCR技术进行12种常见呼吸道病毒的检测。结果在591例标本中病毒阳性率是58%(301/519)。其中呼吸道感染患儿中呼吸道合胞病毒B(RSVB)占首位,为37.5%(113/301),其次是鼻病毒(HRVA/B),为17.3%(52/301),比例最低的是副流感病毒2(PIV2),比例仅为0.7%(2/301)。人偏肺病毒(HMPV)的比例为6%(18/113)。结论 RSVB HRVA/B和PIV3以及Adv是上海市儿童呼吸道疾病感染的主要病原,混合感染以HRVA/B联合其他病毒为主。
Objective To detect the prevalence of respiratory virus infection in children with multiple PCR in Shanghai and get the epidemiology of disease spectrum. Methods From January 2009 to March 2010, 519 samples of lower respiratory tract inpatients with respiratory tract infection were collected from Shanghai Children’s Hospital. Multiplex PCR was used to detect 12 common respiratory viruses. Results The positive rate of virus in 591 specimens was 58% (301/519). Respiratory syncytial virus (RSVB) accounted for 37.5% (113/301) of the children with respiratory tract infection, followed by rhinovirus (HRVA / B), which was 17.3% (52/301), with the lowest proportion Influenza virus 2 (PIV2) at a ratio of only 0.7% (2/301). The proportion of human metapneumovirus (HMPV) was 6% (18/113). Conclusions RSVB HRVA / B, PIV3 and Adv are the major pathogens of respiratory tract infections in children in Shanghai. The majority of combined infections were HRVA / B and other viruses.