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目的:研究牛膝多糖的提取、纯化工艺。方法:采用水煎煮法提取牛膝药材,得粗多糖,经脱蛋白、脱盐和醇沉3步纯化,制备牛膝多糖。以总多糖含量为指标,优选最佳提取纯化工艺。结果:水煎煮的最佳工艺为10倍量水,提取3次,每次2h;脱蛋白工艺中,三氯乙酸(TCA)法优于Sevage法和鞣酸法;脱盐工艺中,分子筛法优于透析法;醇沉使用80%的乙醇。结论:以水煎煮法提取,经脱蛋白、脱盐和醇沉工艺得到的牛膝多糖中总多糖含量及其纯度均较高,可为其药理及制剂研究提供原料。
Objective: To study the extraction and purification of Achyranthes bidentata polysaccharides. Methods: Achyranthes bidentata polysaccharides were extracted by water decoction to obtain crude polysaccharides, which were purified by deproteinization, desalting and alcohol precipitation in three steps to prepare achyranthes bidentata polysaccharides. The total polysaccharide content as an indicator, the best extraction and purification process. Results: The best technology for water decoction is 10 times water, extract 3 times, 2 hours each time; in deproteinization process, TCA method is better than Sevage method and tannic acid method; desalination process, molecular sieve method Better than dialysis; ethanol sink uses 80% ethanol. Conclusion: The content and purity of total polysaccharides in Achyranthes bidentata polysaccharides extracted by decoction and deproteinized, desalted and alcohol precipitation processes are all higher, which can provide raw materials for pharmacology and preparation studies.