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目的旨在通过液体蛋白芯片-MS/MS技术(即CLINPROT技术)发现并鉴定弓形虫感染的血清分子标记物,并分析其应用于临床检测的敏感性和特异性。方法运用CLINPROT技术对弓形虫感染患者的血清样本进行分析,并进一步采用蛋白电泳、质谱鉴定等方法对筛选的蛋白分子进行鉴定,最后通过ELISA方法对其在健康正常人和弓形虫感染患者的血清样品中的表达情况进行了检测,并采用ROC曲线评价了其临床应用价值。结果通过CLINPROT技术,发现了一个在弓形虫感染的血清样本中特异性上调的蛋白,进一步通过蛋白电泳和质谱鉴定方法,发现该特异性上调蛋白为SAA;采用ROC曲线分析显示,其敏感性和特异性分别为84.1%和80%。结论可将CLINPROT技术应用于弓形虫感染血清分子标记物的寻找和鉴定;可将SAA蛋白用作为弓形虫感染的血清分子标志物。
The aim was to discover and identify Toxoplasma gondii infection serum markers by liquid protein chip-MS / MS technology (CLINPROT technique) and to analyze its sensitivity and specificity for clinical testing. Methods CLINPROT was used to analyze serum samples from patients infected with Toxoplasma gondii and the protein molecules were further identified by protein electrophoresis and mass spectrometry. Finally, the protein levels of serum of Toxoplasma gondii infected by Toxoplasma gondii were detected by ELISA. The expression of the sample was tested, and its clinical value was evaluated by ROC curve. Results CLINPROT technique was used to identify a protein that was specifically upregulated in Toxoplasma gondii-infected serum samples. The protein was further identified by SAA and mass spectrometry as SAA. ROC curve analysis showed that its sensitivity and The specificity was 84.1% and 80% respectively. Conclusion The CLINPROT technique can be used to identify and identify serum molecular markers of Toxoplasma gondii infection. SAA protein can be used as a serum molecular marker for Toxoplasma gondii infection.