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本文研究了在 pH =6.70的缓冲溶液 (KH2 PO4 -NaOH)中锌试剂与汞生成的蓝色配合物。在最大吸收处吸光度与汞离子的含量成线性关系 ,配合物的吸收峰值为λmax=545nm ,比锌试剂本身吸收峰值 4 90nm位移 55nm ,配合物的表观摩尔吸光系数εε =2 .8× 10 5L·mol- 1·cm- 1。汞离子的标准工作曲线在 0 .1～ 2 5μg·mL- 1的范围内 ,符合朗伯 -比耳定律。最低检测浓度为 0 .0 1μg·mL- 1,同时 ,研究了测定该配合物的基本条件。实验表明 ,此反应灵敏度较高 ,稳定性和重现性好 ,操作方便 ,可用于环境中的生物体内微量汞的测定。
In this paper, we studied the blue complex formed between zinc reagent and mercury in pH = 6.70 buffer solution (KH2PO4-NaOH). Absorbance at the maximum absorption was linear with the content of mercury ion. The absorption peak of the complex was λmax = 545nm, which was 55nm higher than that of the zinc reagent itself. The apparent molar absorptivity εε = 2. 8 × 10 5L · mol-1 · cm-1. The standard operating curve of mercury ion is in the range of 0 .1 ~ 25μg · mL-1, which accords with Lambert-Beer’s law. The lowest detection concentration was 0. 01μg · mL-1. Meanwhile, the basic conditions for the determination of the complex were studied. Experiments show that the reaction has high sensitivity, good stability and reproducibility, easy operation and can be used for the determination of trace mercury in living organisms.