为研究赤潮藻类与海洋细菌的微生态关系 ,在实验室模拟条件下将塔马亚历山大藻 (Alexandriumtamarense (Lebour)Balech)与海底沉积物中分离的两种细菌 (Bacillusmegaterium ,S7;B .halmapulus,S1 0 )共同培养 ,并探讨了藻菌共培养对A .tamarense生长以及对水体中胞外酶 β 葡萄糖苷酶活性 ( β GlcA)的影响 .实验结果表明 ,在培养的第 2周内 ,与细菌S7或S1 0 共培养的A .tamarense细胞数分别比CK增加约 12 %和 2 4% ,而在第 3周内则各增加 2 3%和 5 %左右 .另外 ,分别与A .tamarense共培养的细菌S7或S1 0 的 β GlcA变化趋势相似 ,呈马鞍型曲线 .在A .tamarense生长的后期 ,β GlcA随着藻细胞的裂解程度的增加而迅速提高 .图 3参 25 In order to study the microecological relationship between red tide algae and marine bacteria, Bacillus megaterium (S7; B.halmapulus, S1) was isolated from Alexandriumtamarense (Lebour) Balech and seafloor sediment under laboratory simulation conditions 0), and discussed the effects of algae co-culture on the growth of A.tamarense and the extracellular enzymeβ-glucosidase activity (β GlcA) in water.The results showed that in the second week of culture, The number of A.tamarense cells co-cultured in S7 or S1 0 was increased by about 12% and 24%, respectively, compared with CK, and increased by 23% and 5% respectively in the third week.In addition, the cells were co-cultured with A.tamarense Of bacterium S7 or S1 0 showed a saddle-shaped curve with a similar trend of change.β GlcA increased rapidly with the increase of the degree of algal cell lysis at the late stage of A. tamarense growth