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目的探讨非小细胞肺癌(Non-Small Cell Lung Cancer,NSCLC)转移抑制基因Kiss-1表达下调或缺失和淋巴管生成与淋巴结转移是否具有协同作用。方法兔抗人单克隆抗体Kiss-1蛋白metastin(45-54)-NH2和抗Podoplanin单克隆抗体(Elivision法)对68例NSCLC术后石蜡切片进行染色,显微镜下观察Kiss-1的表达和肿瘤间质及癌旁组织淋巴管生成情况。结果①NSCLC组织内淋巴结阳性组Kiss-1的表达阳性率为39.4%(13/33),淋巴结阴性组Kiss-1的表达阳性率为91.4%(32/35),两组相比差异有统计学意义(P<0.05);②肿瘤组织间质中淋巴结阳性患者淋巴管密度(Lymphatic vessel density,LVD)平均值为15.73±0.74,淋巴结阴性患者LVD平均值为8.31±0.32,二者相比差异有统计学意义(P<0.05)。③logistic回归分析显示,仅LVD对淋巴结转移有影响(P<0.05),其相对危险度(RR)为2.065,95%可信区间(1.202,3.547)。而Kiss-1的表达下调无意义,P值为0.569,95%可信区间为(0.011,12.143)。二者之间对淋巴结转移无明显协同作用(P<0.05)。结论转移抑制基因Kiss-1的表达下调和淋巴管生成对淋巴结转移未见明显协同作用。
Objective To investigate whether there is a synergistic effect between the expression of Kiss-1 in non-small cell lung cancer (NSCLC) and the expression of Kiss-1 in lymphangiogenesis and lymph node metastasis. METHODS: Sixty-eight paraffin sections of NSCLC were stained with Kissin-1 protein metastin (45-54) -NH2 and anti-Podoplanin monoclonal antibody (Elivision method). The expression of Kiss-1 and tumor Interstitial and adjacent tissue lymphangiogenesis. Results ① The positive expression rate of Kiss-1 in NSCLC tissues was 39.4% (13/33), while the positive expression rate of Kiss-1 in lymph node-negative tissues was 91.4% (32/35). There was statistically significant difference between the two groups (P <0.05). ② The average Lymphatic vessel density (LVD) was 15.73 ± 0.74 in patients with lymph node-positive tumors and 8.31 ± 0.32 in patients with lymph node-negative lymph nodes Statistical significance (P <0.05). Logistic regression analysis showed that LVD only affected lymph node metastasis (P <0.05), and the relative risk (RR) was 2.065, 95% confidence interval (1.202, 3.547). However, the expression of Kiss-1 was not significant, P value was 0.569, 95% confidence interval (0.011,12.143). There was no significant synergistic effect between the two on lymph node metastasis (P <0.05). Conclusion The down-regulation of Kiss-1 and lymphangiogenesis have no obvious synergistic effect on lymph node metastasis.