论文部分内容阅读
将具有肿瘤靶向性的磺胺嘧啶(SD)、二乙三胺五乙酸(DTPA)与葡聚糖(dextran)大分子侧链羟基偶联,合成葡聚糖大分子配体(SD-Dextran-DTPA),再与金属钆离子Gd3+配合,从而制备肿瘤靶向性葡聚糖大分子钆配合物(SD-Dextran-DTPA-Gd).对所合成的配体及钆配合物进行FTIR、UV和1H-NMR等结构表征,测试了配体及钆配合物在水溶液中的粒径分布和zeta电位、钆配合物的体外弛豫率、细胞摄取与T1加权磁共振成像性能.与小分子钆-二乙三胺五乙酸(Gd-DTPA)相比,SD-Dextran-DTPA-Gd具有较高的弛豫率,对肺癌细胞系H460、乳腺癌细胞系MDA-MB-231和T40D均有较好的亲和性,可被肿瘤细胞较好地摄取,并能获得较好的肿瘤细胞磁共振成像.
The SD-Dextran-Ligand (DTPA) and the dextran macromolecule side-chain hydroxyl were coupled with tumor-targeted SD, (DTPA), and then combined with metal gadolinium ion Gd3 + to prepare SD-Dextran-DTPA-Gd. The synthesized ligands and gadolinium complexes were characterized by FTIR, UV and 1H-NMR and other structural characterization, the particle size distribution and zeta potential of the ligand and gadolinium complexes in aqueous solution, the in vitro relaxation rate of gadolinium complexes, cell uptake and T1 weighted magnetic resonance imaging performance were tested.With the small molecular gadolinium - Compared with Gd-DTPA, SD-Dextran-DTPA-Gd had higher relaxation rate, which was better for lung cancer cell line H460 and breast cancer cell lines MDA-MB-231 and T40D Affinity, can be better uptake by tumor cells, and can get better tumor cell magnetic resonance imaging.