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采用酶标抗体竞争结合试验对６株抗ＩＬ－８单克隆抗体识别的表位进行了鉴定。按识别表位的不同可将６株单克隆抗体分为两组，一组包括２Ｇ２、４Ｃ３和４Ｅ１，识别相同或相近的表位；另一组包括４Ｄ７、５Ｃ９和５Ａ４，识别相同或相近的表位。在表位鉴定的基础上，选择识别不同表位的单克隆抗体用于建立双单克隆抗体夹心法ＥＬＩＳＡ检测ＩＬ－８，其中以４Ｄ７为包被抗体，４Ｃ３为酶标抗体的组合可获得最高的敏感性，对单核细胞源性ｒｈＩＬ－８（ＭＩＬ－８）及内皮细胞源性ｒｈＩＬ－８（ＥＩＬ－８）检测的敏感性可分别达到１５６ｐｇ／ｍｌ及３１２ｐｇ／ｍｌ。
The epitopes recognized by the six anti-IL-8 monoclonal antibodies were identified using the enzyme-linked antibody competition binding assay. According to the different epitopes, six monoclonal antibodies can be divided into two groups, one including 2G2, 4C3 and 4E1, which recognize the same or similar epitopes; the other group includes 4D7, 5C9 and 5A4, identifying the same or similar gauge. On the basis of epitope identification, monoclonal antibodies that recognize different epitopes were selected to establish dual sandwich ELISA to detect IL-8. Among them, the combination of 4D7 as coated antibody and 4C3 as ELISA antibody was the highest Sensitivity of 156 pg / ml and 312 pg / ml for the detection of monocyte-derived rhIL-8 (MIL-8) and endothelial-derived rhIL-8 (EIL-8), respectively.