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目的：探讨紫草素(Sh)在人表皮共培养体系中对黑素转运的影响。方法采用MTT法测定紫草素对人黑素细胞（MC）与角质形成细胞（KC）共培养体系增殖的影响，利用荧光标记技术以及流式细胞仪研究紫草素对于黑素转运的影响。结果0.25μmol/L、0.5μmol/L和1μmol/L紫草素对于人表皮共培养体系增殖无影响，0.25μmol/L、0.5μmol/L和1μmol/L紫草素对于共培养体系中黑素转运有剂量依赖地促进作用。结论紫草素对于共培养体系中黑素转运有剂量依赖的促进作用。“,”Objective: To study the Effects of SHIKONIN on cellProliferation and Melanin transfer of Co-Cultured of melanocytes and keratinocytes Methods: MTT method, Flow cytometry were employed to measure the cellviability, melanin transfer of co-cultured melanocytes and keratinocytes Results No influence was observed on co-cultured melanocytes and keratinocytes cellproliferation by 0.25μmol/L, 0.5μmol/L, or 1μmol/L of SHIKONIN . The melanin transfer were clearly enhanced by SHIKONIN in a dose-dependent manner at concentrations of 0.25μmol/L, 0.5μmol/L, and 1μmol/L Conclusion SHIKONIN exhibits a dose-dependent enhancing effects on melanin transfer in co-cultured melanocytes and keratinocytes.