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目的:建立百咳静糖浆的质量控制标准。方法:采用TLC对黄芩、黄柏、陈皮、麻黄进行定性分析,HPLC测定百咳静糖浆中黄芩苷含量。结果:百咳静糖浆供试品与对照品色谱的相应位置上,显相同颜色斑点;Sinochrom ODS bp C18色谱柱(4.6 mm×250 mm,5μm),ODS预柱,流动相甲醇-水-磷酸(45∶55∶0.04),检测波长315 nm,黄芩苷在0.08～0.4μg呈良好线性关系(r=0.999 8),平均回收率100.9%(RSD 1.7%)。结论:该方法灵敏、准确可靠、重复性好,可用于百咳静糖浆的质量控制。
Objective: To establish the quality control standard of Baikejing syrup. Methods: Scutellaria baicalensis, Phellodendron amurense, Citrus rehmanniae and Ephedra were qualitatively analyzed by TLC, and the contents of baicalin in Baikejing syrup were determined by HPLC. Results: The same color spots were observed on the corresponding positions of the sample and the reference substance of Baikejing syrup. Sinochrom ODS bp C18 column (4.6 mm × 250 mm, 5 μm), ODS precolumn, mobile phase methanol-water-phosphoric acid (45:55:0.04). The detection wavelength was 315 nm. The linearity of baicalin was 0.08-0.4 μg (r = 0.999 8). The average recovery was 100.9% (RSD 1.7%). Conclusion: The method is sensitive, accurate and reliable, reproducible, and can be used for the quality control of Baikejing syrup.