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目的:评定LC-MS/MS测定6-甲基巯嘌呤(6-MMP)含量的不确定度。方法:分析LC-MS/MS法测定6-MMP含量的不确定度来源,计算并进行合成和扩展。结果:6-MMP在低含量(2 995 pmol)和高含量(140 900 pmol)的扩展不确定度分别为275.6 pmol和11 396 pmol(P=95%,k=2)。结论:LC-MS/MS法测定人血浆中6-MMP含量的不确定度在低浓度时主要由回收率、基质效应和生物样品配制引入,在高浓度时主要由回收率和生物样品配制引入。
Objective: To assess the uncertainty of LC-MS / MS determination of 6-methyl mercaptopurine (6-MMP) content. Methods: The source of uncertainty for the determination of 6-MMP content by LC-MS / MS method was analyzed, calculated and synthesized. Results: The expanded uncertainty of 6-MMP was 275.6 pmol and 11 396 pmol (P = 95%, k = 2) at low levels (2995 pmol) and high levels (140 900 pmol), respectively. Conclusion: The uncertainty of LC-MS / MS determination of 6-MMP in human plasma at low concentration is mainly caused by the recovery rate, matrix effect and the preparation of biological samples. At high concentration, the uncertainty of 6-MMP content is mainly caused by the recovery rate and the preparation of biological samples .