论文部分内容阅读
14 安瓶启封 首先,仔细检查菌株号码等标记,确保无误。 以酒精棉拭擦安瓶颈部。安瓶内封有棉塞者,用锉对准棉塞的中间部位将玻璃锉一小痕,然后用灭菌纱布或乙烯树脂软片把安瓶颈部裹起来折断。这样做是为了防止由外部吸入杂菌,同时也防止瓶内的干燥菌向外飞散。一般不要用酒精棉包裹折断,因为酒精可能被吸入瓶内。如果玻璃又厚又硬,可先把安瓶颈部在火焰上轻烧一下,立即用酒精棉冷炸,形成裂纹之后就能很容易折断。也可以用烧烫的玻璃棒热炸锉痕。产生裂纹之后,稍放一会儿(半分钟到1分钟),使空气慢慢渗入,以防干燥菌飞散。
14 Ampoule Unpacking First, carefully check the strain number and other marks to ensure correct. Wipe the neck with alcohol cotton swab. Ampoules sealed with tampons who use the file at the middle of the tampon will be a small file glass file marks, and then with sterile gauze or vinyl film wrapped in the neck ampoule broken. This is done to prevent the introduction of bacteria from the outside and to prevent the dry bacteria in the bottle from flying outwards. Generally do not break with alcohol wrapped, because alcohol may be inhaled into the bottle. If the glass is thick and hard, the neck of the ampoule may be first burned in a flame and then immediately cold-fried with alcohol-cotton, which can be easily broken after the crack is formed. You can also heat burned glass rods with hot filing marks. After the crack is generated, let it sit for a while (half a minute to 1 minute), allowing the air to slowly infiltrate to prevent the drying bacteria from scattering.