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目的建立植物油中苯并(a)芘残留的高效液相色谱快速测定法。方法采用CleanertBaP固相萃取小柱对样品进行前处理,高效液相色谱荧光检测测定样品中苯并(a)芘。色谱条件:C18柱(250×4.6 mm,5μm),柱温:35℃,流动相:乙腈-水(82∶18),流速:1.0 ml/min,进样量:20μl,荧光检测器:发射波长406 nm,激发波长384 nm。保留时间定性,峰面积定量。结果方法检出浓度为0.30μg/kg,线性范围2.625μg/L~21.0μg/L,相关系数大于0.999,加标回收率88.7%~94.6%,相对标准偏差均小于5%。结论方法简便、快速、灵敏、重现性好,适于基层推广应用。
OBJECTIVE To establish a rapid method for the determination of benzo [a] pyrene in vegetable oil by high performance liquid chromatography. Methods The samples were pretreated with CleanertBaP solid phase extraction cartridges, and benzo (a) pyrene was determined by high performance liquid chromatography with fluorescence detection. Chromatographic conditions: C18 column (250 × 4.6 mm, 5 μm), column temperature: 35 ° C, mobile phase of acetonitrile-water (82:18), flow rate of 1.0 ml / min, injection volume of 20 μl, fluorescence detector: Wavelength 406 nm, excitation wavelength 384 nm. Retention time qualitative, peak area quantitative. Results The linear range was 2.625μg / L ~ 21.0μg / L. The correlation coefficient was greater than 0.999. The recoveries were 88.7% ~ 94.6%. The relative standard deviations were all less than 5%. Conclusion The method is simple, rapid, sensitive and reproducible. It is suitable for popularization and application of grassroots.