1 材料与方法 1.1 检测对象 1998年1月～12月,来院就诊的慢性乙型肝炎伴有肝功能酶学异常的患者303例,采取血清检测HBV-M及HBV-DNA并列入分析。 1.2 试剂与方法 HBV-M采用ELISA法检测,HBV-DNA用PCR方法采用1109型微电脑自动基因扩增仪检测,所用试剂均由华美生物技术公司提供,严格按说明书操作;ALT、AST试剂为北京中生生物工程公司提供,用日本AU560全自动生 1 Materials and Methods 1.1 test subjects from January 1998 to December, to hospital treatment of chronic hepatitis B patients with abnormal liver enzymes 303 patients, serum HBV-M and HBV-DNA were detected and included in the analysis. 1.2 Reagents and methods HBV-M using ELISA detection, HBV-DNA PCR method using 1109 microcomputer automatic gene amplification instrument detection, reagents used by the gorgeous biotechnology company, in strict accordance with the instructions; ALT, AST reagent for Beijing In the bio-engineering company to provide, with Japan AU560 automatic students