目的:观察六君子汤对中性粒细胞活化刺激传导系统的第二信使——细胞内Ca~(2+)浓度以及细胞内pH值、环磷酸腺苷(cAMP)浓度变化的影响,以研究六君子汤抑制中性粒细胞活化的作用机理。 方法:①六君子汤提取物颗粒以磷酸缓冲液(PBS,pH 7.4)混悬,用超声波击碎颗粒,60℃加热30min后过滤,调整至适宜浓度(10～100μg/ml)。②以比重离 Objective: To observe the effects of Liujunzi decoction on the second messenger of neutrophil activation and stimulating the conductive system: intracellular Ca~(2+) concentration, intracellular pH value and cyclic adenosine monophosphate (cAMP) concentration change. The Action Mechanism of Liujunzi Decoction on Neutrophil Activation. Method: 1 Six Gentlemen Decoction extract particles were suspended in phosphate buffer (PBS, pH 7.4), and the particles were disrupted by ultrasonic wave, heated at 60°C for 30 minutes, filtered, and adjusted to an appropriate concentration (10 to 100 μg/ml). 2 by the proportion of