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依据牛肝菌属卷边组Boletus sect.Appendiculati内7个物种的核糖体基因rDNA内转录间隔区(internal transcribed spacer,ITS)序列比对,设计5对ITS特异引物,分别用于卷边牛肝菌Boletus appendiculatus与亚卷边牛肝菌B.subappendiculatus、卷边牛肝菌与拟桃红牛肝菌B.pseudoregius、华靛牛肝菌B.roseoflavus与卷边牛肝菌B.appendiculatus、华靛牛肝菌与华美牛肝菌B.speciosus、拟桃红牛肝菌与华美牛肝菌的相互识别。ITS区段的PCR扩增结果表明,5对ITS特异引物皆成功扩增出可用于辨别这些近缘物种的目的条带。但未能设计出ITS特异引物,以识别华靛牛肝菌与桃红牛肝菌B.regius两个近缘物种。
Five pairs of ITS specific primers were designed according to the rDNA internal transcribed spacer (ITS) sequences of seven species of Boletus sect. Appendiculati in the curl group, B. Boletus appendiculatus and B. subappendiculatus, B. curcumae and B. pseudoregius, B. roseoflavus and B. appendiculatus, Liver and Bolete B.speciosus, Peppermint and Porcini Mutual Recognition. The result of PCR amplification of ITS region showed that 5 pairs of ITS specific primers successfully amplified the target bands that could be used to distinguish these relatives. However, ITS-specific primers were not designed to identify two relatives B. bovis and B. bosius.