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甲胎蛋白(AFP)不仅是诊断原发性肝癌的比较可靠指标之一,而且也可参照它预测异常胎儿。为了使其操作手续适应新的发展之需要,我们对之进行了一些改进。 经过实验,选择了抗体最佳浓度是1:20,000(最终反应浓度)。分离剂为22.5％的PEG(M=12,000)最终浓度达7.5％。标记抗原量定为5,000 CPM/分/管。除了PEG 100微升外,其它试液(包括标准液和正常兔新鲜血清)均为50微升。确定了上述条件后,我们进行了保温时间的选择。于37℃水浴中分别保温15、30、45、60、120、180分钟,再分别加入22.5％CPG 100微升,
Alpha-fetoprotein (AFP) is not only one of the more reliable indicators of primary liver cancer diagnosis, but also can be used to predict abnormal fetus. In order to adapt its operating procedures to the needs of new developments, we made some improvements. After the experiment, the optimal concentration of the selected antibody is 1: 20,000 (final reaction concentration). The final concentration of PEG (M = 12,000) with 22.5% separation agent was 7.5%. The amount of labeled antigen was set at 5,000 CPM / min / tube. Except for PEG 100 μl, all other test solutions (both standard and normal rabbit fresh serum) were 50 μl. After determining the above conditions, we conducted a selection of holding time. Were incubated at 37 ℃ water bath for 15, 30, 45, 60, 120, 180 minutes, respectively, and then were added 22.5% CPG 100 μl,