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目的:建立测定消栓通颗粒中黄芪甲苷含量的新方法。方法:采用高效液相色谱-蒸发光散射检测法测定,色谱柱为kromasilC18柱,以乙腈-水(32:68)为流动相,流速为1.0ml/min,ELSD条件为飘逸管温度为105℃,载气流速为2.5L/min;测定消栓通颗粒中黄芪甲苷的含量。结果:黄芪甲苷在1.08μg-6.48μg范围内与峰面积线性关系良好(r=0.9999),平均加样回收率为100.2%,RSD为0.93%。结论:方法灵敏、可靠、准确、重复性好,可作为消栓通颗粒的质量控制方法。
Objective: To establish a new method to determine the content of astragaloside IV in Xiaoshuan Tong Granules. METHODS: A HPLC-evaporative light scattering detection method was used. The column was a kromasil C18 column with acetonitrile-water (32:68) as the mobile phase, the flow rate was 1.0 ml/min, and the ELSD condition was 105°C. The carrier gas flow rate was 2.5L/min; the content of astragaloside IV in Xiaoshuantong granules was determined. Results: The linear relationship between astragaloside IV and peak area was good in the range of 1.08μg-6.48μg (r=0.9999). The average recovery was 100.2% and the RSD was 0.93%. Conclusion: The method is sensitive, reliable, accurate, and reproducible. It can be used as a quality control method for Xiaosuantong particles.