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目的:检测PD-L1基因的常规和可变剪接变异体mRNA在胃癌组织和正常胃组织中的表达。方法:以RT-PCR方法从胃癌和正常胃组织的总RNA中扩增PD-L1的cDNA,并将扩增产物通过测序鉴定。结果:4例胃癌标本中,从病例2中扩增到常规和可变剪接变异体,但以常规剪接体为主,病例3仅扩增到常规剪接体PD-L1Ⅰ,病例4扩增到其可变剪接变异体PD-L1Ⅱ,而病例1两种剪接形式均未扩增到。在2例正常胃组织中均只扩增到可变剪接变异体PD-L1Ⅱ。结论:PD-L1基因在大多数胃癌组织中表达,正常与胃癌组织中还表达可变剪接变异体PD-L1Ⅱ,提示表达产物的剪接方式具有多样性。
Objective: To detect the expression of PD-L1 gene in normal and alternative splicing variant mRNA in gastric cancer and normal gastric tissue. Methods: The cDNA of PD-L1 was amplified by RT-PCR from the total RNA of gastric cancer and normal gastric tissue, and the amplified product was identified by sequencing. Results: Four cases of gastric cancer samples were amplified from case 2 to conventional and alternative splicing variants, but the conventional splice variant was the main one. Case 3 only amplified to PD-L1Ⅰ, and case 4 amplified to The alternative splicing variant PD-L1II, but not both of the two spliced forms of case 1, was amplified. In 2 normal gastric tissues, only the variant splicing variant PD-L1Ⅱ was amplified. Conclusion: PD-L1 gene is expressed in most of gastric cancer tissues. PD-L1Ⅱ is also expressed in normal and gastric cancer tissues, suggesting that the splicing patterns of the expressed products are diverse.