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目的制备艰难梭菌菌体特异性鸡卵黄抗体,探讨其对艰难梭菌感染小鼠的治疗作用。方法建立艰难梭菌相关腹泻(CDAD)小鼠模型,将30只CDAD小鼠随机分为5组,每组6只,以11.86mg/mL、1.186mg/mL、0.1186mg/mL艰难梭菌菌体特异性IgY灌胃,设立空白对照组及阴性对照组,1d/次,0.5mL/只,连续3d。末次治疗24h后处死小鼠,取盲肠组织中段制作病理切片;其余盲肠组织进行DAO、D-乳酸和TNF-α含量测定;测定回肠组织含水率。结果阴性对照组和空白对照组小鼠盲肠黏膜脱落伴大量炎细胞浸润;高IgY组小鼠盲肠黏膜完整,未见炎性细胞浸润;中IgY组小鼠盲肠黏膜少量炎细胞浸润;低IgY组小鼠盲肠黏膜上皮细胞脱落,少量炎细胞浸润。高IgY组小鼠盲肠DAO含量均显著高于空白对照组和阴性对照组(P<0.01);中IgY组小鼠DAO含量高于阴性对照组(P<0.05);中、高IgY组小鼠TNF-α含量均低于低IgY组、空白对照组和阴性对照组(P<0.05)。结论艰难梭菌菌体特异性IgY对艰难梭菌感染小鼠具有治疗作用。
Objective To prepare C. cholerae-specific chicken egg yolk antibody and investigate its therapeutic effect on C. difficile-infected mice. Methods A mouse model of C. difficile-associated diarrhea (CDAD) was established. Thirty CDAD mice were randomly divided into five groups with 6 mice in each group, with 11.86 mg / mL, 1.186 mg / mL and 0.1186 mg / mL C. difficile Body specific IgY gavage, the establishment of a blank control group and a negative control group, 1d / time, 0.5mL / only, continuous 3d. Mice were sacrificed 24 h after the last treatment, and the middle part of cecum was used to make pathological sections. The contents of DAO, D-lactic acid and TNF-α in the other cecal tissues were determined. The water content of ileal tissue was measured. Results The negative control group and the blank control group had cecal mucosal detachment accompanied by a large number of inflammatory cell infiltration. The cecum mucosa of high IgY group was intact with no infiltration of inflammatory cells. Small amount of inflammatory cell infiltration of cecal mucosa in IgY group mice and low IgY group Mice cecal mucosal epithelial cells shedding, a small amount of inflammatory cell infiltration. The content of DAO in the high IgY group was significantly higher than that in the blank control group and the negative control group (P <0.01). The DAO content in the middle IgY group was higher than that in the negative control group (P <0.05) TNF-α content were lower than low IgY group, blank control group and negative control group (P <0.05). Conclusion Clostridium difficile mycobacterial-specific IgY has a therapeutic effect on C. difficile-infected mice.