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采用PCR法扩增来自国内5个不同产地的裂叶荆芥ITS全序列,测序后以产自河北的裂叶荆芥代表中国裂叶荆芥与来自不同国家的裂叶荆芥ITS序列进行比较,构建分子系统发育树,探讨国内5个不同产地及不同国家的裂叶荆芥的亲缘关系和系统进化。结果表明:国内5个不同产地的裂叶荆芥ITS1和ITS2序列均有较高的G/C含量;5个产地的裂叶荆芥的扩增序列长度均为749bp,且序列完全相同;其中ITS1序列长231bp,5.8S序列长168bp,ITS2序列长236bp。中国裂叶荆芥与日本、韩国裂叶荆芥ITS序列一致性为100%,与美国裂叶荆芥ITS序列一致性为99.0%。与美国裂叶荆芥相比,中国裂叶荆芥ITS序列有7个碱基发生变异。来自不同国家的裂叶荆芥形成单系群和2个分支(中、日、韩3国为1个分支,美国单独形成1个分支)。ITS序列的一致性表明国内5个不同产地裂叶荆芥为同一个种。
The ITS sequence of Nepeatus intermedius was amplified by PCR from five different habitats in China. After sequencing, the ITS sequences of Nepeta schlegeli from China were compared with that of Nepeta schlegeli from different countries to construct molecular phylogenetic tree Domestic 5 different origin and different countries Crataegus Schizocacenetic and phylogenetic relationship. The results showed that ITS1 and ITS2 sequences of Nepeta schizonepeta had higher G / C content in five different habitats in China. The sequences of Nepalese were all 749bp in length and the sequences were exactly the same. The sequence of ITS1 was 231bp , 5.8S sequence length 168bp, ITS2 sequence length 236bp. The correspondence between ITS and ITS sequences of Neperismans schizonepeta japonica and Japan and South Korea was 100% and 99.0%, respectively. Compared with the American Nepeta schizonepeta, the nucleotide sequence of ITS in Chinese Nepeta schizonepeta has a mutation of 7 bases. Nepeta clematis from different countries formed a monophyletic group and two branches (China, Japan, South Korea as a branch of 3 countries, the United States formed a separate branch). The consistency of ITS sequences indicates that the five species of Nepeanula in different parts of China are the same species.