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目的建立大鼠实验性精索静脉曲张（experimental varicocele EV）的模型，测量睾丸中过氧化氢酶（catalase CAT）活性和Bcl-2的表达，并分析两者的变化关系。方法将30只雄性青春期Wistar大鼠随机分为EV8周组和12周组（各9只）和相应的假手术对照组2组（各6只），通过部分结扎左肾静脉建立大鼠EV模型，分别于术后8周、12周处死动物，测左侧精索静脉直径，用比色法测CAT活力，免疫组化法测Bcl-2的表达。结果成功建立了EV模型，与相应的对照组相比左侧精索静脉直径明显增大（P<0.01）。E V组双侧睾丸的C A T活性显著低于相应的对照组（P<0.01），左侧睾丸比右侧睾丸更低，但无明显统计学意义（P＞0.05）。EV组双侧睾丸间质细胞中Bcl-2的染色指数与相应的对照组相比均显著降低（P<0.01），左侧睾丸染色指数比右侧睾丸下降更明显（P<0.01）， EV12周组与EV8周组相比，EV12周组染色指数更低（P<0.05）。CAT活性与Bcl-2的染色指数在0.01水平有显著相关性。结论我们对EV模型做了观察和研究，认为过氧化氢酶通过抑制氧化应激可能影响Bcl-2并抑制细胞凋亡。“,”Objective To establish experimental varicocele (EV) model in rats to measure the catalase (CAT) activity and the Bcl-2 expression in the testes, and analyze the variation of CAT and Bcl-2.Methods The varicocele model was created by partial ligation of the left renal vein in adolescent male Wistar rats.A total of 30 male adolescent Wistar rats were randomly divided into 8-week EV group, 12-week EV group and the corresponding sham-operated control group. The rats were kil ed 8 weeks and 12 weeks after operation, the histology of the testis was studied, the activity of CAT was determined by Colorimetry and the Bcl-2 was determined by Immunohistochemistry. Results The experimental varicocele model was successful y created. Compared with the corresponding control group, the diameter of the left spermatic vein enlarged remarkably(P<0.001). The CAT activity of the bilateral testes in EV groups was decreased significantly compared with that in the corresponding control groups (P0.05). The staining indices of Bcl-2 in bilateral testicular mesenchymal cells of the EV groups decreased significantly compared with that in the corresponding control group (P<0.01), and the left sides decreased more significantly than the right sides (P<0.01), the staining indices decreased more significantly in EV12 group than that in EV8 group (P<0.05). The correlation between the activity of CAT and the staining indices of Bcl-2 is significant at the 0.01 level. Conclusion We made an observation and research on EV model, considered that CAT may affect Bcl-2 and inhibit apoptosis by inhibiting the oxidative stress.