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Objective:The aim of this study was to determine the expression of Hoxa 10 in endometrium during the menstrual cycle and their response to sex steroids and HB-EGF.Methods:Forty endometrial samples from regularly cycling women were studied.The endometrial epithelial(EEC)and stromal(ESC)cells isolated by collagenase Ⅰ digestion and mechanical dissociation was cultured from every sample.The endometrial stromal cells(ESC)were incubated with 17-beta estradiol(10-8mol/L),medroxyprogesterone acetate(MPA)(10-6mol/L),RU486(10-8mol/L)or HB-EGF(10ng/ml)for 48 hours respectively.The expression of Hoxa10 mRNA was demonstrated by reverse transcription-polymerase chain reaction(RT-PCR).Result:Hoxa10 showed a regulated cycle phase-dependent expression pattern in stromal cells and epithelial cells.Hoxa10 expression dramatically increased during the midsecretory phase of the menstrual cycle,at the time of implantation.The expression of Hoxa10 in cultured endometrial stromal cells was stimulated by estrogen,progesterone and HB-EGF.Conclusion:The cycle phase-specific expression of Hoxa10 and up-regulated by sex steroids and HB-EGF suggests a tight regulation and establishing conditions necessary for implantation.
Objective: The aim of this study was to determine the expression of Hoxa 10 in endometrium during the menstrual cycle and their response to sex steroids and HB-EGF. Methods: Forty endometrial samples from regularly cycling women were studied.The endometrial epithelial (EEC) and stromal (ESC) cells isolated by collagenase I digestion and mechanical dissociation was cultured from every sample. The endometrial stromal cells (ESC) were incubated with 17-beta estradiol (10-8 mol / L), medroxyprogesterone acetate (MPA) The expression of Hoxa10 mRNA was demonstrated by reverse transcription-polymerase chain reaction (RT-PCR). Result: Hoxa10 (10ng / ml) showed a regulated cycle phase-dependent expression pattern in stromal cells and epithelial cells. Hoxa10 expression dramatically increased during the midsecretory phase of the menstrual cycle, at the time of implantation. expression of Hoxa10 in cultured endometrial stromal cells was stimulated by est rogen, progesterone and HB-EGF.Conclusion: The cycle phase-specific expression of Hoxa10 and up-regulated by sex steroids and HB-EGF suggests a tight regulation and establishing conditions necessary for implantation.