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目的观察肿瘤抑制基因ｐ１６、ｐ５３及细胞周期依赖激酶抑制物ｐ２１基因单独或联合应用时对肺癌细胞增殖的影响。方法应用十八酰基胺阳离子脂质体介导ｐ１６、ｐ５３、ｐ２１基因单独或共转染到非小细胞肺癌细胞系Ａ５４９和小细胞肺癌细胞系ＳＨ７７，观察转染后１、３、５日该细胞增殖的活力。采用四甲基偶氮唑盐微量酶反应比色法（ＭＴＴ法）测定吸光度（Ａ），以检测细胞增殖活力。结果Ａ５４９细胞系：Ａ均值分别为：空白组０．７８５６，脂质体组０．７４７８，ｐ１６组０．５５８９，ｐ２１组０．６０２２，ｐ５３组０．５７７８，ｐ１６＋ｐ２１组０．４７１１，ｐ１６＋ｐ５３组０．３０１７，实验组与对照组比较Ｐ＜０．０１，以共转染ｐ１６＋ｐ５３组最强。ＳＨ７７细胞系：Ａ均值分别为：空白组１．０２２２，脂质体组０．９３１６，ｐ１６组０．９１３７，ｐ２１组０．８８８９，ｐ５３组０．８３５６，ｐ１６＋ｐ２１组０．５９３３，ｐ１６＋ｐ５３组０．４２７８，单转染实验组与对照组比较Ｐ＞０．０５；共转染实验组与对照组比较Ｐ＜０．０１，以ｐ１６＋ｐ５３组更明显。结论ｐ１６、ｐ５３和ｐ２１基因有可能成为非小细胞肺癌基因替代治疗的候选基因，联合应用ｐ１６与ｐ２１基?
Objective To observe the effect of tumor suppressor gene p16, p53 and cell cycle dependent kinase inhibitor p21 gene on lung cancer cell proliferation when used alone or in combination. Methods The p16, p53, and p21 genes mediated by octadecylamine cationic liposomes alone or co-transfected into the non-small cell lung cancer cell line A549 and the small cell lung cancer cell line SH77 were observed on days 1, 3, and 5 after transfection. The viability of cell proliferation. The absorbance (A) was measured by MTT method with MTT to detect cell viability. Results A549 cell lines: A mean values were: blank group 0.7856, liposome group 0.7478, p16 group 0.5589, p21 group 0.6022, p53 group 0.5778, p16+p21 group 0.4711, p16+p53 group 0.3017, the experimental group compared with the control group P <0.01, the most co-transfected p16 + p53 group. SH77 cell lines: A mean values were: blank group 1.0222, liposome group 0.9316, p16 group 0.9137, p21 group 0.8889, p53 group 0.8356, p16+p21 group 0.5933, p16+p53 group 0 .4278, P>0.05 in the single transfection experimental group compared with the control group; P<0.01 in the co-transfection experimental group compared with the control group, and more obvious in the p16+p53 group. Conclusion The p16, p53 and p21 genes may be candidate genes for gene replacement therapy for non-small cell lung cancer.