Cuprous oxide (Cu2O) has attracted plenty of attention for potential nonlinear photonic applications due to its superior third-order nonlinear optical property such as two-photon absorption.In this paper,we investigated the two-photon excita-tion induced carrier dynamics of a Cu2O thin film prepared by radio-frequency magnetron sputtering,using the femtosecond transient absorption experiments.Biexponential dynamics including an ultrafast carrier scattering (＜ 1 ps) followed by a carrier recombination (＞ 50 ps) were observed.The time constant of carrier scattering under two-photon excitation is larger than that under one-photon excitation,due to the different transition selection rules and smaller absorption coefficient of the two-photon excitation.