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用聚合酶链反应技术(ＰＣＲ)极量稀释法定量检测乙型肝炎病毒(ＨＢＶ)ＤＮＡ,探讨ＨＢＶ感染剂量在评价ＨＢｓＡｇ携带者血液传染性方面的价值。从连续入院的992例外科病人检出ＨＢｓＡｇ阳性者156例,对其ＨＢｅＡｇ和ＨＢＶＤＮＡ进行检测,将套式ＰＣＲ检ＨＢＶＤＮＡ低?..
Quantitative detection of Hepatitis B virus (HBV) DNA by the method of polymerase chain reaction (PCR) using an extremum dilution method was used to evaluate the value of HBV infection in assessing the blood transfusion of HBsAg carriers. 156 cases of HBsAg positive patients were detected from 992 cases of consecutive hospitalized patients, HBeAg and HBVDNA were detected by nested PCR.