论文部分内容阅读
为有效控制人参保健大米的质量,以7种人参皂苷(Rg1,Re,Rb1,Rc,Rb2,Rd,Rf)为指标,研究人参皂苷成分的检测方法。结果表明:在色谱柱为ACQUITY UPLC BEH C18色谱柱(2.1×50mm,1.7μm),流动相乙腈-水梯度洗脱(0~5min,15%~19%乙腈;5~7min,19%~20%乙腈;7~9min,20%~25%乙腈;9~13min,25%~38%乙腈;13~13.5min,38%~60%乙腈;13.5~14min,48%~15%乙腈),柱温35℃,流速0.4mL/min,检测波长203nm,进样量2μL的条件下进行检测,7种人参皂苷的线性范围分别为Rg1:5.62~81.0μg/mL,Re:5.18~259.0μg/mL,Rb:14.80~240.0μg/mL,Rc:4.70~235.0μg/mL,Rb:26.26~313.0μg/mL,Rd:5.83~291.5μg/mL,Rf:2.09~104.5μg/mL,其相关系数为0.999 5~0.999 9,线性关系良好,加样回收率为98.8%~100.2%。样品中7种人参皂苷(Rg1,Re,Rf,Rb1,Rc,Rb2,Rd)的含量分别为0.017 2mg/g、0.019 6 mg/g、0.028 8 mg/g、0.042 6 mg/g、0.023 4 mg/g、0.016 4 mg/g和0.035 6mg/g。该方法简便,分离效果好,精确度高,可用于该产品的质量控制。
In order to effectively control the quality of ginseng health care rice, seven kinds of ginsenosides (Rg1, Re, Rb1, Rc, Rb2, Rd, Rf) were taken as indexes to study the method of detecting ginsenosides. The results showed that the mobile phase consisted of ACQUITY UPLC BEH C18 column (2.1 × 50 mm, 1.7 μm), mobile phase acetonitrile-water gradient (0-5 min, 15-19% acetonitrile, 5-7 min, 19-20% % Acetonitrile; 7 to 9 min, 20% to 25% acetonitrile; 9 to 13 min, 25% to 38% acetonitrile; 13 to 13.5 min, 38% to 60% acetonitrile; 13.5 to 14 min, 48% to 15% The linear range of the seven ginsenosides were Rg1: 5.62 ~ 81.0μg / mL, Re: 5.18 ~ 259.0μg / mL, the detection wavelength was set at 203nm and the injection volume was 2μL. , Rb: 14.80-240.0 μg / mL, Rc: 4.70-235.0 μg / mL, Rb: 26.26-313.0 μg / mL, Rd: 5.83-291.5 μg / mL, Rf: 2.09-104.5 μg / mL, and the correlation coefficient was 0.999 5 ~ 0.999 9, good linearity, sample recovery was 98.8% ~ 100.2%. The contents of seven kinds of ginsenosides (Rg1, Re, Rf, Rb1, Rc, Rb2 and Rd) in the sample were 0.017 2 mg / g, 0.019 6 mg / g, 0.028 8 mg / g, 0.042 6 mg / g and 0.023 4 mg / g, 0.016 4 mg / g and 0.035 6 mg / g. The method is simple, the separation effect is good, the precision is high, and the quality control of the product can be used.