桂花种子休眠期较长,当年的种子一般要沙藏到第二年春天才能发芽。采用组织培养的方法,可以打破种子的休眠。选用当年成熟的种子,常规消毒,然后剥取完整的成熟胚接种到MS附加0.5—2毫克/升6BA培养基上,在温度27℃和光照1000勒克斯的条件下培养。4天后可见胚轴、子叶开始 Osmanthus seeds longer dormancy, when the seeds of the sand generally to the next spring to germinate. Tissue culture methods can break seed dormancy. The mature seeds of the current year were selected and routinely sterilized. The whole mature embryos were then stripped and inoculated into MS supplemented with 0.5-2 mg / L 6BA medium and cultured at 27 ° C and 1000 lux. Hypocotyls were seen after 4 days, cotyledons began