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以美国红叶紫薇优株当年生半木质化枝条为外植体,采用丛芽发生途径,建立其组培快繁技术体系。结果表明,最适腋芽诱导培养基为MS+6-BA 1.0 mg·L-1+IBA 0.05 mg·L-1,诱导率高达88%;最佳增殖培养基为ZW(改良的WPM)+6-BA 0.5mg·L-1+IBA 0.05 mg·L-1,增殖系数可达5.21,芽高4.6 cm;生根最适培养基为ZW+IBA 0.5 mg·L-1,生根率100%,平均每株苗生根4~5条。炼苗后,移栽于泥炭土和珍珠岩3:1(V/V)的混合基质中,成活率高达95%以上。
Using the semi-lignified shoots of the American red leaf crape myrtle excellent strain as explants, using the way of bud-bud generation, the tissue culture technique system was established. The results showed that the optimal induction medium for axillary buds was MS + 6-BA 1.0 mg · L-1 and IBA 0.05 mg · L-1, the induction rate was as high as 88%. The best proliferation medium was ZW (modified WPM) +6 -BA 0.5 mg · L-1 and IBA 0.05 mg · L-1, the proliferation coefficient was 5.21 and the shoot height was 4.6 cm. The optimum medium for rooting was ZW + IBA 0.5 mg · L-1, the rooting rate was 100% 4 ~ 5 rootlets per plant. After the seedling was harvested, the survival rate was higher than 95% after being transplanted in a mixed matrix of 3: 1 (V / V) peat soil and perlite.