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目的:获得辣椒(Capsicum annuum)G蛋白β亚基基因(CaArcA)序列。方法:以拟南芥同源序列为探针,利用电子克隆技术获得了CaArcA基因的cDNA全长,采用生物信息学方法,对该基因所编码的蛋白从理化性质、分子进化、高级结构等多角度进行分析。结果:该基因cDNA全长1 173 bp,其开放阅读框为993 bp,编码330个氨基酸,分子量为36.1 kDa;具有23个磷酸化位点,无信号肽和跨膜区域,定位在细胞膜外。通过同源比对发现,CaArcA蛋白与与拟南芥(Arabidopsis thaliana)的同源蛋白相似性在80%以上。结论:利用电子克隆的方法获得一个辣椒G蛋白β亚基基因的cDNA序列。
Objective: To obtain the Ca protein sequence of the G protein β subunit of Capsicum annuum. Methods: Using the Arabidopsis thaliana homologous sequence as a probe, the full-length cDNA of CaArcA gene was obtained by electronic cloning technique. The bioinformatics method was used to analyze the protein encoded by this gene from physical and chemical properties, molecular evolution and higher structure Angle analysis. RESULTS: The full-length cDNA of this gene was 1 173 bp in length and contained an open reading frame of 993 bp encoding a protein of 330 amino acids with a molecular mass of 36.1 kDa. The cDNA had 23 phosphorylation sites, no signal peptide and transmembrane domain and was located outside the cell membrane. By homology comparison, it was found that the similarity of CaArcA protein and homologous protein with Arabidopsis thaliana was more than 80%. Conclusion: The cDNA sequence of G protein β subunit gene was obtained by electronic cloning method.