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目的:观察联合检测尿液四项微量蛋白质的临床意义。方法:采用全定量酶免疫测定法(EIA)分别检测126例肾病病人及30例正常人的尿微量白蛋白(micro-albumin,mAlb)、视黄醇结合蛋白(retinoi-binding protein,RBP)、β2-微球蛋白(β2-microglobulin,β2-MG)及免疫球蛋白G(immunoglobulin G,IgG)的含量,并将两者的检测结果进行比较。结果:①IgG:糖尿病性肾病组、高血压性肾病组和慢性肾炎组显著高于其他组(P<0.01);②mAlb:除高血压组和对照组无显著差异(P>0.05)外,其他观察组均明显高于对照组(P<0.01);③β2-MG:糖尿病组、糖尿病性肾病组和高血压性肾病组均明显高于对照组和其他观察组(P<0.01);④RBP:糖尿病组、糖尿病性肾病组和高血压性肾病组均明显高于对照组和其他观察组(P<0.01)。结论:mAlb、RBP、β2-MG可作为监测早期糖尿病肾病和高血压性肾病较敏感指标,尿微量蛋白联合检测对早期肾损伤有重要意义。
Objective: To observe the clinical significance of combined detection of four trace proteins in urine. Methods: Urinary micro-albumin (mAlb), retinoi-binding protein (RBP) were detected in 126 patients with nephropathy and 30 normal controls by enzyme-linked immunosorbent assay (EIA) β2-microglobulin (β2-MG) and immunoglobulin G (IgG) were measured and compared with each other. Results: ①IgG: diabetic nephropathy group, hypertensive nephropathy group and chronic nephritis group were significantly higher than the other groups (P <0.01); ② mAlb: except hypertension group and control group no significant difference (P> 0.05), other observations (P <0.01); ③β2-MG: diabetes mellitus group, diabetic nephropathy group and hypertensive nephropathy group were significantly higher than the control group and other observation groups (P <0.01); ④RBP: diabetic group , Diabetic nephropathy group and hypertensive nephropathy group were significantly higher than the control group and other observation groups (P <0.01). Conclusion: mAlb, RBP and β2-MG can be used as sensitive indicators for monitoring early diabetic nephropathy and hypertensive nephropathy. Combined detection of urinary microalbumin is of great significance for early renal injury.