紫外线诱变育种已成为食用菌育种的有效方法之一,但对诱变机理方面的研究甚少。本试验旨在研究紫外线诱变对平菇菌丝长速、生长势和细胞内含物变化的影响,为食用菌紫外线诱变育种提供参考和依据。 一、材料与方法 (一)供试材料 菌株为无孢5号,本校分离。平板培养基为综合马铃薯汁培养基:去皮马铃薯200g,葡萄糖20g,琼脂20g,磷酸二氢钾38,硫酸镁1.5g,维生素B_1 1片,水1000ml,pH值自然。接种后将平板置恒温箱25℃下培养。 (二)照射剂量与方法 试验设1′30″、2′、3′、5′和对照5个处理,每处理重复3次,每重复用18×90mm培养皿平板培养菌丝,每个平板中央接入8mm的菌丝 UV mutagenesis breeding has become one of the effective methods of edible fungus breeding, but little research on mutagenesis mechanism. The purpose of this experiment is to study the effect of UV mutagenesis on the growth, growth potential and changes of cell contents of Pleurotus ostreatus mycelia, and provide reference and basis for the mutagenesis of mushroom. First, the material and methods (a) for the test material strain No spore No. 5, the school separation. Plate medium for the integrated potato juice medium: peeled potato 200g, glucose 20g, agar 20g, potassium dihydrogen phosphate 38, magnesium sulfate 1.5g, vitamin B_1 1 tablets, water 1000ml, pH value natural. After inoculation, the plates were incubated in an incubator at 25 ° C. (B) irradiation dose and method test set 1’30 ", 2 ’, 3’, 5 ’and control 5 treatment, each treatment repeated 3 times, each repeated 18 × 90mm petri dish culture mycelium plate, each plate Central access to 8mm mycelium