Polycrystalline SrTiO3 thin films with the cubic perovskite structure were grown on quartz substrates by pulsed laser deposition. The first-order Raman scattering processes, which were forbidden in SrTiO3 single crystals, were observed in the films at 300 K and lower temperatures due to the structural distortion causing by strain effect and oxygen vacancies. The polar TO2 phonon showed a typical Fano asymmetry in the entire temperature region from 95K to 300K. In contrast, the nonpolar TO3 phonon and polar TO4 phonons were mostly symmetric.